Calcium Chloride Transformation
From Wikipedia, the free encyclopedia
- The correct title of this article is CaCl2 Transformation. It appears incorrectly here because of technical restrictions.
 |
This article is orphaned as few or no other articles link to it. Please help introduce links in articles on related topics. (March 2008) |
CaCl2 transformation refers to a technique of increasing the ability of a cell to incorporate plasmid DNA. The addition of CaCl2 to a cell suspension promotes the binding of plasmid DNA to the cell surface, which can then pass into the cell. CaCl2 transformation is also accompanied by heat shock, where cells are cooled to a low temperature (~4 degrees Celsius) and then heated to a high temperature (~42 degrees Celsius) for a short time.
