Bromobimane

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Bromobimane
IUPAC name	3-(bromomethyl)-2,5,6-trimethyl-1H,7H- pyrazolo[1,2-a]pyrazole-1,7-dione
Other names	Bromobimane, mBBr
Identifiers
CAS number	[71418-44-5]
PubChem	24885999
Properties
Molecular formula	C10H11BrN2O2
Molar mass	271.11 g mol-1
Melting point	152–154 °C
Solubility in water	in MeOH, DMF, DMSO
Hazards
Main hazards	alkylating agent
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references

Bromobimane is a heterocyclic compound that is used as a reagent in biochemistry. It alkylates thiol groups, replacing the H with a fluorescent tag (λ_emission = 478 nm). Its alkylating properties are comparable to iodoacetamide.^[1]

[edit] References

1. ^ Paul C. Chinn, Vincent Pigiet, and Robert C. Fahey (1986). "Determination of thiol proteins using monobromobimane labeling and high-performance liquid chromatographic analysis: Application to Escherichia coli thioredoxin". Analytical Biochemistry 159: 143–149. doi:10.1016/0003-2697(86)90319-2.