Auramine-rhodamine stain

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Oocysts of Cryptosporidium parvum stained with the fluorescent auramine-rhodamine stain.
Oocysts of Cryptosporidium parvum stained with the fluorescent auramine-rhodamine stain.

The auramine-rhodamine stain (AR), also known as the Truant auramine-rhodamine stain, is a histological technique used to visualizee acid-fast bacilli using fluorescence microscopy, notably species in the Mycobacterium genus.[1] Acid-fast organisms display a reddish-yellow fluorescence.[2]

AR stain is a mixture of Auramine O and Rhodamine B.

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[edit] References

  1. ^ Kommareddi S, Abramowsky C, Swinehart G, Hrabak L (1984). "Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis". Hum Pathol 15 (11): 1085-9. doi:10.1016/S0046-8177(84)80253-1. PMID 6208117. 
  2. ^ Abe C (2003). "[Standardization of laboratory tests for tuberculosis and their proficiency testing]". Kekkaku 78 (8): 541-51. PMID 14509226. 
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