ATP-dependent DNA helicase 2 subunit 2

From Wikipedia, the free encyclopedia

X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa)

PDB rendering based on 1jeq.

Available structures: 1jeq, 1jey, 1q2z, 1rw2

Identifiers

Symbol(s)

XRCC5; FLJ39089; KARP-1; KARP1; KU80; KUB2; Ku86; NFIV

External IDs

OMIM: 194364 MGI: 104517 HomoloGene: 40681

EC number

3.6.1. EC 3.6.1.

Gene ontology
Molecular Function:	• nucleotide binding • molecular_function • DNA binding • double-stranded DNA binding • ATP-dependent DNA helicase activity • helicase activity • protein binding • ATP binding • hydrolase activity
Cellular Component:	• cellular_component • nucleus • cytoplasm • DNA-dependent protein kinase complex
Biological Process:	• regulation of DNA repair • double-strand break repair via nonhomologous end joining • DNA recombination

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_021141 (mRNA)
NP_066964 (protein)

NM_009533 (mRNA)
NP_033559 (protein)

Location

Chr 2: 216.68 - 216.78 Mb

Chr 1: 72.24 - 72.33 Mb

Pubmed search

[1]

[2]

X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa), also known as XRCC5, is a human gene.

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.^[1]

[edit] References

^ Entrez Gene: XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa).

[edit] Further reading

Koike M (2003). "Dimerization, translocation and localization of Ku70 and Ku80 proteins.". J. Radiat. Res. 43 (3): 223–36. PMID 12518983.
Chen DJ, Park MS, Campbell E, et al. (1992). "Assignment of a human DNA double-strand break repair gene (XRCC5) to chromosome 2.". Genomics 13 (4): 1088–94. PMID 1505945.
Wedrychowski A, Henzel W, Huston L, et al. (1992). "Identification of proteins binding to interferon-inducible transcriptional enhancers in hematopoietic cells.". J. Biol. Chem. 267 (7): 4533–40. PMID 1537839.
Jeggo PA, Hafezparast M, Thompson AF, et al. (1992). "Localization of a DNA repair gene (XRCC5) involved in double-strand-break rejoining to human chromosome 2.". Proc. Natl. Acad. Sci. U.S.A. 89 (14): 6423–7. PMID 1631138.
Knuth MW, Gunderson SI, Thompson NE, et al. (1990). "Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter.". J. Biol. Chem. 265 (29): 17911–20. PMID 2211668.
Stuiver MH, Coenjaerts FE, van der Vliet PC (1990). "The autoantigen Ku is indistinguishable from NF IV, a protein forming multimeric protein-DNA complexes.". J. Exp. Med. 172 (4): 1049–54. PMID 2212941.
Mimori T, Ohosone Y, Hama N, et al. (1990). "Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome.". Proc. Natl. Acad. Sci. U.S.A. 87 (5): 1777–81. PMID 2308937.
Yaneva M, Wen J, Ayala A, Cook R (1989). "cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.". J. Biol. Chem. 264 (23): 13407–11. PMID 2760028.
Genersch E, Eckerskorn C, Lottspeich F, et al. (1995). "Purification of the sequence-specific transcription factor CTCBF, involved in the control of human collagen IV genes: subunits with homology to Ku antigen.". EMBO J. 14 (4): 791–800. PMID 7882982.
Tuteja N, Tuteja R, Ochem A, et al. (1994). "Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen.". EMBO J. 13 (20): 4991–5001. PMID 7957065.
Le Romancer M, Reyl-Desmars F, Cherifi Y, et al. (1994). "The 86-kDa subunit of autoantigen Ku is a somatostatin receptor regulating protein phosphatase-2A activity.". J. Biol. Chem. 269 (26): 17464–8. PMID 8021251.
Cao QP, Pitt S, Leszyk J, Baril EF (1994). "DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen.". Biochemistry 33 (28): 8548–57. PMID 8031790.
Taccioli GE, Gottlieb TM, Blunt T, et al. (1994). "Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination.". Science 265 (5177): 1442–5. PMID 8073286.
Chen DJ, Marrone BL, Nguyen T, et al. (1994). "Regional assignment of a human DNA repair gene (XRCC5) to 2q35 by X-ray hybrid mapping.". Genomics 21 (2): 423–7. doi:10.1006/geno.1994.1287. PMID 8088837.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. PMID 8125298.
Kaczmarski W, Khan SA (1993). "Lupus autoantigen Ku protein binds HIV-1 TAR RNA in vitro.". Biochem. Biophys. Res. Commun. 196 (2): 935–42. doi:10.1006/bbrc.1993.2339. PMID 8240370.
Blunt T, Taccioli GE, Priestley A, et al. (1996). "A YAC contig encompassing the XRCC5 (Ku80) DNA repair gene and complementation of defective cells by YAC protoplast fusion.". Genomics 30 (2): 320–8. PMID 8586433.
Warriar N, Pagé N, Govindan MV (1996). "Expression of human glucocorticoid receptor gene and interaction of nuclear proteins with the transcriptional control element.". J. Biol. Chem. 271 (31): 18662–71. PMID 8702520.
Myung K, He DM, Lee SE, Hendrickson EA (1997). "KARP-1: a novel leucine zipper protein expressed from the Ku86 autoantigen locus is implicated in the control of DNA-dependent protein kinase activity.". EMBO J. 16 (11): 3172–84. doi:10.1093/emboj/16.11.3172. PMID 9214634.
Jin S, Kharbanda S, Mayer B, et al. (1997). "Binding of Ku and c-Abl at the kinase homology region of DNA-dependent protein kinase catalytic subunit.". J. Biol. Chem. 272 (40): 24763–6. PMID 9312071.