2-methylisocitrate dehydratase

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In enzymology, a 2-methylisocitrate dehydratase (EC 4.2.1.99) is an enzyme that catalyzes the chemical reaction

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate $\rightleftharpoons$ (Z)-but-2-ene-1,2,3-tricarboxylate + H₂O

Hence, this enzyme has one substrate, (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate, and two products, (Z)-but-2-ene-1,2,3-tricarboxylate and H₂O.

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]. This enzyme is also called (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate hydro-lyase. This enzyme participates in propanoate metabolism.

[edit] References

IUBMB entry for 4.2.1.99
BRENDA references for 4.2.1.99 (Recommended.)
PubMed references for 4.2.1.99
PubMed Central references for 4.2.1.99
Google Scholar references for 4.2.1.99
Aoki H, Uchiyama H, Umetsu H, Tabuchi T (1995). "Isolation of 2-methylisocitrate dehydratase, a new enzyme serving in the methylcitric acid cycle for propionate metabolism, from Yarrowia lipolytica". Biosci. Biotechnol. Biochem. 59: 1825–1828.
Tabuchi T, Umetsu H, Aoki H, Uchiyama H (1995). "Characteristics of 2-methylisocitrate dehydratase, isolated from Yarrowia lipolytica, in comparison to aconitase". Biosci. Biotechnol. Biochem. 59: 2013–2017.